Kinetic modelling of 2,3-butanediol production by Raoultella terrigena CECT 4519 resting cells: Effect of fluid dynamics conditions and initial glycerol concentration

Biodiesel-derived glycerol was biologically converted to 2,3-butanediol, an organic compound with multiple industrial applications. Recent studies showed that Raoultella terrigena CECT 4519 is effective biocatalyst for the bioprocess under conventional growing conditions. In present work, a novel composed by R. resting cells evaluated first time. Fluid dynamic conditions has been optimized maximize 2,3-butanediol production in terms of titre, yield, and selectivity. Regarding effect initial concentration batch runs, no substrate inhibition detected studied (concentrations between 45 250 g/L were used). Employing pure as carbon source, 82.0 titre achieved, whereas 76.5 reached using raw glycerol. These numbers involve achieved yield respect maximal theoretic 79% 77%, respectively. A successful kinetic modelling developed it able describe both evolution concentrations relevant components time rates calculated at experimental values. Estimated specific rate 0.034 g2,3-BDO/gX·h, while estimated empirical pseudo-stoichiometric coefficient glycerol/2,3-butanediol 2.52 gGly/g2,3-BDO. The obtained results promising biocatalyst, which provides new opportunities developing scaling-up biorefineries.